11: The study of the effect of ozone on the metabolic state of rat spinal cord neurons

Topic: Basic Science
Subtopic: Pain category (ICD-11), Chronic neuropathic pain, , , , , , , , , , Pharmacological interventions

Background and Aims: Metabonomics analysis was used to detect the changes of the metabolic status of primary spinal cord nerve cells after medical ozone treatment, and to verify the differential expression of a series of metabolic molecules in primary spinal cord nerve cells caused by ozone.The possible molecular mechanisms and pathways of neurotoxicity of medical ozone were studied by means of information biology analysis and statistical methods.

Methods: The cultured rat spinal cord cells were divided treatment group( 40μg/ mL ozone ) and control group. The effect of ozone on the expression level of metabolic molecules was detected by liquid chromatography-mass spectrometry.Multivariate Analysis was carried out by informobiology technology, and PCA was used to identify the quality of the data.Metaboalalyst software was used for univariate analysis to obtain the metabolic molecular differences after ozone exposure. The Metlin database was used for biomarker identification. The degree, distribution and related metabolic pathways of metabolic molecular differences were observed by generating volcanic maps, cluster heat maps and enrichment analysis maps.

Results: The metabolic molecules in treatment group was significantly changed compared with control group, and the total peak area was significantly lower than that in control group .
In positive mode, PC1 contained 46.0% cumulative variance, followed by PC2 13.9%. In negative mode, PC1 contained 43.6% cumulative variance, followed by PC2 15.3%
In positive mode, there are two principal components, R2X=0.419, R2Y=0.993, Q2=0.886;In negative mode, R2X=0.469, R2Y=0.994, Q2=0.945.
A total of 104 metabolites were differentially expressed by volcanic map.The heat maps showed that 25 differences changed the intra-group and inter-group differences of metabolic molecules.

Conclusions: The expression level of metabolic molecules in spinal cord of primary rats treated with 40μg/ mL medical ozone was significantly different from that of control group.The levels of ADP, AMP, aconic acid, UDP-glucose, NAM, L-glutamine, riboflavin and other metabolic molecules were differentially changed, mainly involving fatty acid, tricarboxylic acid cycle, purine metabolism, niacin and niacinamide metabolism, amino acid, lipid metabolism, riboflavin metabolism pathway.

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Presenting Author: Tao Sun
Submitting Author: Zhijian Fu

Senior Author: no

IASP 2026 Global Year theme of Neuropathic Pain: Yes